Effect of incubation with cytomix on O2 consumption by Caco-2 enterocytes. (a) In some studies, O2 consumption was measured after cells growing on microcarrier beads were incubated for 24 hours with either control medium (Control24; n = 11) or medium containing cytomix (Cyto24; n = 12). (b) In additional experiments, enterocytes that had been exposed to cytomix for 24 hours were washed extensively, and then incubated for a further 4 hours with either cytokine-free medium (Cyto24/Control4; n = 9) or cytomix-containing medium (Cyto24/Cyto4; n = 4). Data were analyzed using Student's t test. *P < 0.05 versus Control24, †P < 0.05 versus Cyto24/Control4. Reprinted from  with permission.