Table 1 The sequences of the primers used for qPCR analysis
From: Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein
Gene | Â | Primer sequences |
|---|---|---|
Human ACE2 | Forward | 5'-GATAGTGGTTGGCATTGTC-3' |
Human ACE2 | Reverse | 5'-CGATGGAGGCATAAGGAT -3' |
Human TBP | Forward | 5'-ATCAGTGCCGTGGTTCGT-3' |
Human TBP | Reverse | 5'-TTCGGAGAGTTCTGGGATTG-3' |
Mouse p22phox | Forward | 5'-CATCAAGCAACCACCTAC -3' |
Mouse p22phox | Reverse | 5'-CATCTGTCACTGGCATTG -3' |
Mouse p47phox | Forward | 5'-ACACCTTCATTCGCCATA -3' |
Mouse p47phox | Reverse | 5'-CTGTAGACCACCTTCTCC -3' |
Mouse p67phox | Forward | 5'-TTGAAGAAGGGCAGTGAT -3' |
Mouse p67phox | Reverse | 5'-TTAGGAGGTGGTGGAATATC -3' |
Mouse Rac1 | Forward | 5'-TCATCAGTTACACGACCAA-3' |
Mouse Rac1 | Reverse | 5'-CGCAATCTGTCATAATCTTCT-3' |
Mouse Rac2 | Forward | 5'-ATGTGATGGTGGACAGTAA-3' |
Mouse Rac2 | Reverse | 5'-TAGCGAGAAGCAGATGAG-3' |
Mouse NOX1 | Forward | 5'-GGTGTCTTGCTTGATAATCTT-3' |
Mouse NOX1 | Reverse | 5'-CTTGGTCGTTCTATGTTGTT-3' |
Mouse NOX2 | Forward | 5'-CAAGAACATTACCTTCCACAT-3' |
Mouse NOX2 | Reverse | 5'-TGATATAGCCAGTCCAACC-3' |
Mouse NOX3 | Forward | 5'-GTGTGGTATAAGTGTTGTGAA-3' |
Mouse NOX3 | Reverse | 5'-TAAATGAGCCTTCCCTTGT-3' |
Mouse NOX4 | Forward | 5'-TCAGTGAACTACAGTGAAGAT-3' |
Mouse NOX4 | Reverse | 5'-GGAATGGTTGTGAAGAGAAG-3' |
Mouse NOX5 | Forward | 5'-TACTACCTGGACATCACTAAC-3' |
Mouse NOX5 | Reverse | 5'-CTTGGAGAATGGCTAGGAT-3' |
Mouse Duox1 | Forward | 5'-TTCCTCAACCGCTACATT-3' |
Mouse Duox1 | Reverse | 5'-GTGGACTGATGGAGAACA-3' |
Mouse Duox2 | Forward | 5'-GAAGACTGCTCAAGATGTG-3' |
Mouse Duox2 | Reverse | 5'-TCAATCACTATCCTGTCCTC-3' |
Mouse TBP | Forward | 5'-ATCAGTGCCGTGGTTCGT-3' |
Mouse TBP | Reverse | 5'-TTCGGAGAGTTCTGGGATTG-3' |
Mouse GAPDH | Forward | 5'-AACCTGCCAAGTATGATGA-3' |
Mouse GAPDH | Reverse | 5'-GGAGTTGCTGTTGAAGTC-3' |