Fig. 7

The effect of APOH administration on macrophage polarization. a Flow cytometry was employed to determine the change in the M1 and M2 polarization in PMs. b–c The percentage of M1- and M2-polarized PMs was detected. d Flow cytometry was employed to determine the change in the M1 and M2 polarization in RAW 264.7 macrophages. e–f The percentage of M1- and M2-polarized RAW 264.7 cells were detected. g Western blot analysis of iNOS and Arg-1 in PMs. h–i Relative expression levels of iNOS and Arg-1 in PMs. j Western blot analysis of iNOS and Arg-1 in RAW 264.7 cells. k–l Relative expression levels of iNOS and Arg-1 in RAW 264.7 cells. The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. *p < 0.05, **p < 0.01, *** p < 0.001, **** p < 0.0001. Apolipoprotein H, APOH; peritoneal macrophages, PMs; lipopolysaccharide, LPS; inducible nitric oxide synthase, iNOS; Arginase 1, Arg-1