Fig. 8

APOH inhibits the TLR4/NF-κB pathway in macrophage cells treats with LPS. a The mRNA expression levels of TLR4, MyD88, TRAF6, JNK and P65 in PMs. b–e Western blotting analysis of the relative expression of the TLR4, MyD88, and TRAF6 proteins in PMs. f–g Western blot analysis of the expression of phosphorylated JNK in PMs. h–i Western blot analysis of the phosphorylated P65 in PMs. j The mRNA expression of TLR4, MyD88, TRAF6, JNK and P65 in RAW 264.7 cells. k–n Western blotting analysis of the relative expression of TLR4, MyD88, and TRAF6 proteins in RAW 264.7 cells. o–p Western blot analysis of the expression of phosphorylated JNK in RAW 264.7 cells. q–r Western blot analysis of the expression of phosphorylated p65 in RAW 264.7 cells. Primer sequences in mRNA were listed in Additional file 5: Table S2. The data are presented as the means ± standard deviations (S.D.). “*” indicates a difference between groups. *p < 0.05, **p < 0.01, *** p < 0.001, **** p < 0.0001. Apolipoprotein H, APOH; peritoneal macrophages, PMs; lipopolysaccharide, LPS; Toll-like receptor 4, TLR4; myeloid differentiation factor 88, MyD88; nuclear factor-κB, NF-κB; TNF receptor associated factor 6, TRAF6; Jun N-terminal kinase, JNK